Serena and Venus Williams' [ Images ] stranglehold of women's doubles was ended at Wimbledon [ Images ] on Wednesday when the American sisters were knocked out in the quarter-finals by Vera Zvonareva [ Images ] and Elena Vesnina.
A 3-6, 6-3, 6-4 defeat to the Russian duo ended Venus's tournament after she suffered a shock defeat to Bulgaria's world number 82 Tsvetana Pironkova [ Images ] in the last eight of the women's singles on Tuesday.
The Williams' title at the French Open [ Images ] earlier this month was their 12th together in Grand Slams and meant they held all four majors at the same time.
The top seeds and champions here for the past two years had not dropped a set on their way to the quarters but Venus sent a return into the net to seal their fate and end an awful couple of days for the 30-year-old.
Zvonareva is also in the singles semi-final against Pironkova after beating former world number one Kim Clijsters [ Images ] on Tuesday.
Serena is left to fly the flag for the Williams family in the other women's semi-final against Petra Kvitova [ Images ].