Barca coach Pep Guardiola warned their high scoring victory in Spain last week could bring the best out of the German champions, who have called the defeat a debacle and said they have no real chance of qualifying.
"Bayern are capable of scoring four goals, all the more so when they are wounded," Guardiola said at a news conference.
"I don't believe the statements about them already having given the tie up for lost," he said.
"I am sure they will put in a good performance and we have to go there with a lot of ambition to win the match. If we are speculative they will roll over us."
Guardiola left key players, including midfielder Xavi [ Images ], Primera Liga top scorer Samuel Eto'o and captain Carles Puyol [ Images ], out of his starting line-up for Saturday's comfortable 2-0 home win over struggling Recreativo Huelva.
The former Barca and Spain midfielder will have to watch the match from the stands after being dismissed from the bench in the first leg and seeing his appeal with UEFA [ Images ] rejected.
"I'll be in the stands but it does not matter, it is something completely secondary," he said.
Barca central defender Rafael Marquez [ Images ] is suspended after picking up a yellow card at the Nou Camp.
Bayern, who had not lost in the Champions League this season before last week's defeat, have said the team has an obligation to restore the club's pride.
Boosted by their 4-0 crushing of Eintracht Frankfurt on Saturday, the Germans are looking to bow out with their heads held high.
"With this win (against Frankfurt) we repaired part of the damage but not all of it. We have a lot to do on Tuesday so that we can say goodbye to the competition in a good way," coach Juergen Klinsmann said.
Klinsmann is again expected to field 34-year-old keeper Hans Joerg Butt, ahead of Michael Rensing, who had been his first choice throughout the season until last week.
"Unfortunately I have to hurt Michael and put his development on the backburner so we can get the results we need to get," the coach, in his first season at Bayern, said.
Klinsmann, under fire for his team's erratic performances throughout the season, will have a largely fit squad with only central defender Daniel van Buyten in doubt.
Bayern Munich [ Images ]: 22-Hans Joerg Butt; 23-Massimo Oddo, 6-Martin Demichelis, 3-Lucio, 21-Philipp Lahm; 8-Hamit Altintop, 31-Bastian Schweinsteiger, 17-Mark van Bommel, 15-Ze Roberto, 7-Franck Ribery; 9-Luca Toni
Barcelona: 1-Victor Valdes; 20-Daniel Alves, 3-Gerard Pique, 5-Carles Puyol, 16-Silvinho; 6-Xavi, 24-Yaya Toure, 8-Andres Iniesta; 10-Lionel Messi, 9-Samuel Eto'o, 14-Thierry Henry.
Referee: Roberto Rosetti (Italy [ Images ])